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Comparative cytotoxicity of CHA-ETO, HA-ETO and ETO in MCS. (A) MIA PaCa-2 MCS (treatment 48 h, 37°C); P<0.05, CHA-ETO vs. ETO, and P<0.05, CHA-ETO vs. HA-ETO. (B) Dose-dependent cytotoxicity of CHA-ETO in MIA PaCa-2 and MCF-7 MCS (48 h, 37°C). (C) Time-dependent cytotoxicity of CHA-ETO in MIA PaCa-2 and MCF-7 MCS (drug concentration 25 μg/mL); P<0.05, MIA PaCa-2 vs. MCF-7. Cytotoxicity was determined using LDH assay. Data are shown as means ± SEM (n = 5).
