Table 1.
Cytokines in the supernatants of whole-SCC tumor specimen culture
| Sample No. | IFN-γ (pg/ml) | IL-1β (pg/ml) | IL-2 (pg/ml) | IL-6 (pg/ml) | IL-8 (pg/ml) | TNF-α (pg/ml) | IL-12p70 (pg/ml) | GM-CSF (pg/ml) | IL-10 (pg/ml) | VEGF (pg/ml) | TGF-β1 (pg/ml) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| TSN 1 | 6.3 | 17.8 | 3.2 | 211.8 | 10985.6 | 15.8 | 1.2 | 39.7 | 13.6 | 164.9 | 38.8 |
| TSN 2 | N.D. | 76.1 | 5.9 | 162.9 | 11098.0 | 34.8 | N.D. | 43.9 | 25.1 | 545.2 | 31.5 |
| TSN 3 | N.D. | 43.8 | 1.8 | 2821.1 | 10704.9 | N.D. | 1.3 | 480.3 | 6.3 | 1489.2 | 67.7 |
| TSN 4 | N.D. | N.D. | N.D. | N.D. | 3.0 | N.D. | N.D. | N.D. | N.D. | 103.0 | 20.3 |
| TSN 5 | 8.0 | 2.8 | 0.1 | 9.6 | 282.9 | N.D. | N.D. | N.D. | N.D. | 231.5 | 46.7 |
| TSN 6 | 8.0 | 0.2 | N.D. | 0.9 | 145.4 | N.D. | N.D. | N.D. | N.D. | 94.2 | 11.0 |
| TSN 7 | N.D. | 1.1 | 5.1 | 108.6 | 14948.5 | N.D. | 0.5 | 62.9 | 5.6 | 763.6 | 21.9 |
| TSN 8 | N.D. | 26.3 | 4.8 | 510.7 | 12005.4 | 41.4 | 0.5 | 171.6 | 21.4 | 706.0 | 26.5 |
| TSN 9 | N.D. | 30.2 | 5.3 | 530.4 | 11517.7 | N.D. | N.D. | 64.1 | 20.1 | 171.2 | 20.9 |
| TSN 10 | N.D. | 25.8 | 6.2 | 107.8 | 6815.9 | 14.6 | 0.3 | 53.7 | 8.3 | 398.6 | 44.1 |
| TSN 11 | 16.8 | 3.1 | 0.7 | 17.8 | 479.0 | N.D. | 1.9 | 10.9 | 3.5 | 110.2 | 22.0 |
| TSN 12 | N.D. | 20.2 | 7.6 | 469.3 | 12037.7 | 10.2 | 1.1 | 49.9 | 6.2 | 117.1 | 28.1 |
| Media 1 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | 11.0 |
| Media 2 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | 10.1 |
| Media 3 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | 11.1 |
Size comparable specimens of whole-tissue SCC specimens were cultured for 24 hours, and the contents of IFN-γ, IL-1β, IL-2, IL-6, IL-8, IL-12p70, TNF-α, GM-CSF, IL-10, VEGF, and TGF-β1 in the supernatants were analyzed using electrochemiluminescence-based method. Media 1-3 represent the media used for the culture of SCC specimens. TSN, tumor supernatant. N.D., Not detected.