Table 2.
Preclinical therapeutic studies of stem cells in sepsis and ALI/ARDS
| Author | Date | Cell Type | Disease | Clinical Outcome With Stem Cells |
|---|---|---|---|---|
| Rojas et al | 2005 | Bone marrow-derived MSC | Bleomycin-induced ALI | Histologic decrease in lung injury Increase in GM-CSF and G-CSF Decrease in proinflammatory cytokines |
| Gupta et al | 2007 | Bone marrow-derived MSC | Endotoxin induced ALI | Histologic decrease in lung injury Increase survival Decrease in excess lung water and BAL protein Decrease proinflammatory cytokines Increase anti-inflammatory cytokine |
| Xu et al | 2007 | Bone marrow-derived MSC | Endotoxin induced ALI | Histologic decrease in lung injury Decrease in excess lung water Decrease proinflammatory cytokines Increase anti-inflammatory cytokines |
| Kahler et al | 2007 | Bone marrow-derived EPC (cultured in vitro) | ALI | Integration of EPC into tissue of injured lung |
| Ortiz et al | 2007 | Bone marrow-derived MSC and MSC-conditioned medium | Bleomycin-induced ALI | Decrease proinflammatory cytokines Decrease BAL protein |
| Zhao et al | 2008 | Bone marrow-derived MSC | Bleomycin-induced ALI | Histologic decrease in lung injury Decrease in laminin and hyaluronan in BAL Decrease proinflammatory cytokines |
| Lam et al | 2008 | Peripheral blood EPC (cultured in vitro) | ALI | Histologic decrease in lung injury Decrease endothelial dysfunction in pulmonary artery Decrease excess lung water |
| Nemeth et al | 2008 | Bone marrow-derived MSC | Sepsis | Improve survival and organ dysfunction Decrease proinflammatory cytokines Increase anti-inflammatory cytokines |
| Gonzalez-Rey et al | 2009 | Adipose-derived MSC | Sepsis | Histologic and clinical decrease in colitis Improve survival and organ dysfunction Decrease proinflammatory cytokines Increase anti-inflammatory cytokines |
| Lee et al | 2009 | Human allogeneic MSC and human MSC-conditioned medium | ALI | Histologic decrease in lung injury Decrease excess lung water Restored alveolar fluid clearance |
| Mei et al | 2010 | Bone marrow-derived MSC | Sepsis | Histologic decrease in lung injury Decrease BAL protein Improve survival in setting of antibiotics Improved organ dysfunction Decrease proinflammatory cytokines |
| Gene plus cell therapy | ||||
| Mei et al | 2007 | Syngeneic MSC with and without ANGPT1 gene | Endotoxin induced ALI | Histologic decrease in lung injury Decrease in inflammatory cells in BAL Near complete reversal of increased lung permeability with gene transfection |
| Xu et al | 2007 | Bone marrow derived MSC with and without Ang1 gene | Endotoxin induced ALI | Further histologic decrease in lung injury with gene transfection vs. MSC alone Further decrease excess lung water and BAL protein vs. MSC alone Further decrease in proinflammatory cytokines |
ALI, acute lung injury; ARDS, acute respiratory distress syndrome; FACS, fluorescence-activated cell sorting; MSC, mesenchymal stromal cell; EPC, endothelial progenitor cell; GM-CSF, granulocyte–monocyte colony-stimulating factor; BAL, bronchoalveolar lavage.