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Photobleaching of GFP-tagged hHV1 occurs in two distinct irreversible steps, indicating that the channel exists mainly as a dimer. A: blue circles indicate individual channels that were followed over time. B: time course of fluorescence intensity of one spot (i.e., channel). C: illustrates that most channels bleached in two steps. [From Tombola et al. (497), with permission from Elsevier.]
