Fig. 4.

Following WA treatment, an array of proteins associated with the Akt/mTOR and MAPK growth, proliferation, and survival pathways as well as surface membrane proteins that signal to each pathway were screened via Western blotting in U87 and U251 cells for total expression and activation via phosphorylation status at 24h post-exposure in order to help evaluate the molecular changes corresponding with cellular observations. Alterations in expression are observed in both pathways and with the cell surface receptors