Skip to main content
. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Biochem J. 2012 Aug 15;446(1):89–98. doi: 10.1042/BJ20111942

Figure 7.

Figure 7

Physiological interaction of USF1 with the E-box of GATA5 promoter in mouse intestinal mucosa. The lysate of cross-linked intestinal mucosa was immunoprecipitated with either anti-USF1 or anti-USF2 antibody or without any antibody. Cross-links were reversed and the protein was digested with proteinase K. The recovered DNA was PCR-amplified using the primers listed in Supplementary Table 1 and products were analyzed by agarose gel electrophoresis. A: The location of the primers (arrows) relative to the E-box at −118 to −113 is shown. B: The E-box binding site was PCR-amplified with –258For/+85Rev primers and confirmed by sequencing. C: Quantitative PCR analysis was performed in duplicate for each CHIP experiment. The fold enrichment of USF1 and USF2 antibodies at E-Box promoter region over a non-promoter region of GATA5 gene was calculated. All qChIP data were normalized to non-immune serum control. Significant difference of enrichment between USF1 and USF2 antibodies was observed (P < 0.05).