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. 2013 Jun 10;8(6):e65120. doi: 10.1371/journal.pone.0065120

Figure 3. The genomic organization, protein localization and transcriptional activation activity of the TaWRKY10 gene.

Figure 3

(A) Southern blot analysis of the TaWRKY10 gene. 10 µg genomic DNA of hexaploid wheat cv. Chinese Spring was digested completely with the restriction enzymes. The ORF of TaWRKY10 was used as the hybrid probe. The TaWRKY10 overexpressing vector was used as control. The 1 kb DNA Ladder (MBI Fermentas) are indicated on the left. (B) Subcellular localization of TaWRKY10 in onion epidermal cells. Onion epidermal cells were transferred with vector carrying GFP or TaWRKY10-GFP using bombardment method. Free GFP and TaWRKY10-GFP fusion proteins were transiently expressed in onion epidermal cells and observed with an inverted fluorescence microscope. (C) Transactivation activity of the TaWRKY10 protein in Yeast. The schematic diagram demonstrating the TaWRKY10 cDNA fragments encoding different portions of TaWRKY10 that were fused to the yeast vector pGBKT7 (pBD). Transactivation activity analysis of TaWRKY10 was performed using yeast strain AH109. The transformants were streaked on the SD/−Trp or on SD/−His medium. The transformants were examined for growth in the presence of 3-AT and X-α-D-gal. Three biological experiments were carried out, which produced similar results.