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. 2013 Jun 10;8(6):e65403. doi: 10.1371/journal.pone.0065403

Figure 3. Stimulation of Hedgehog-primed Daoy cells with EGF induced downregulation of canonical HH/GLI targets.

Figure 3

Daoy cells were either treated with control medium (purple), Shh-N conditioned medium (blue), EGF (red) or a combinatorial treatment with Shh-N medium and EGF (green) treated for 24 h. (A) Transcript dynamics for GLI1, PTCH, and HHIP were analyzed by transcript profiling on whole genome arrays. Curves represent mean values of independent biological experiments (n = 3, +/−SEM). (B) Taqman RT-PCR confirmed downregulation of canonical Hedghog targets by EGF.