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Samples were obtained after inducing EGFR signaling in Shh-N primed Daoy cells (Shh-N) or unprimed Daoy cells (EGF) for 3 h or 18 h with EGF (A) or AREG (B). Control cells were neither primed with SAG nor exposed to EGF. Western blots were probed with antibodies against GLI1, GLI3 and HSP70. Figure S6 A shows GLI3A/GLI3R ratios for crosstalk induced by EGF and AREG.
