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| Control | EGF | Shh-N | Shh-N/EGF | |
| pMEK1/2 | 0 | up | 0 | up |
| pERK1/2 | 0 | up | 0 | up |
| pAKT (S473) | 0 | up | 0 | up |
| pp70S6K | 0 | 0 | 0 | 0 |
| pGSK3ß | 0 | 0 | 0 | 0 |
| pRSK1–3 | 0 | 0 | 0 | 0 |
| pPLCγ (Y771) | 0 | 0 | 0 | 0 |
| pPLCγ (Y783) | 0 | 0 | 0 | 0 |
| pcJUN (S63) | 0 | up | 0 | up |
| pcJUN (S73) | 0 | up | 0 | up |
| E-Cadherin | 0 | up | 0 | up |
| pmTOR (S2448) | 0 | 0 | 0 | 0 |
| pRPS6 (S235/236) | 0 | up | 0 | up |
| pCREB (S133) | 0 | 0 | 0 | 0 |
| pRB (S807/811) | 0 | 0 | 0 | 0 |
| pNFκB (S276) | 0 | 0 | 0 | 0 |
