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. 2013 Jun 10;8(6):e67054. doi: 10.1371/journal.pone.0067054

Figure 6. Fibulin-2 promotes cross-linking of secreted collagen and tumor cell adherence to collagen.

Figure 6

(A) Compressive testing performed on punch biopsies of un-injected lungs (n=13) and 344SQ orthotopic lung tumors (Fbln2 shRNA [n=9] or SCR shRNA [n=5] transfectants). Results represent mean values ± S.D. of replicate samples. (B) Quantification of pepsin-soluble and total collagen in conditioned media samples by sircol assays and hydroxyproline assays, respectively. Results represent mean values ± S.D. of triplicate samples. (C) Quantitative RT-PCR assays on 344SQ cells stably transfected with scrambled control shRNA (SCR) or Fbln2 shRNA #1. Values normalized on the basis of ribosomal protein L32 mRNA and expressed as the mean values (± S.D.) of quadruplicate samples. P-values are indicated (t-test). (D) Adherent cells were stained with crystal violet and quantified colorimetrically 1 h after seeding on plastic or coated with indicated matrix molecules or bovine serum albumin (BSA) as a control. Results expressed as mean values (± S.D.) of triplicate wells. (E) Quantitative RT-PCR assays on 344SQ cells stably transfected with scrambled control shRNA (SCR) or Fbln2 shRNA #1. Values normalized on the basis of ribosomal protein L32 and expressed as the mean values (± S.D.) of quadruplicate samples. P-values are indicated (t-test).