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. 2013 Jun 10;8(6):e66825. doi: 10.1371/journal.pone.0066825

Figure 1. Viability and permissiveness of glioma cells to MYXV infection.

Figure 1

Alamar blue cell viability assay was used to determine the percentage of viable U87 (A) and U251 (B) cells following 24 and 48 h infection with MYXV at the indicated MOI. Columns represent means of triplicate of one representative experiment. Error bars represent 95% confidence intervals, MOI =  multiplicity of infection. (C) Flow cytometric analysis to determine percentage of cells infected with MYXV. MYXV used contained GFP under the control of a poxvirus synthetic early-late promoter insert and located between the open reading frame M135R and M136R of the MYXV genome. GFP expression (detected on the FLI channel) indicates number of cells infected with MYXV (97.8% and 96.7% respectively for U87 and U251 cells). Mock-infected cells were used to set the gating. MYXV Infection was for 18 h. A representative of at least 5 independent experiments is shown. MYXV =  Myxoma virus, GFP =  green fluorescent protein.