Skip to main content
. 2013 Jun 10;8(6):e66825. doi: 10.1371/journal.pone.0066825

Figure 5. MYXV down-regulates MHC I via M153R.

Figure 5

Dot plot showing the role of M153R in HLA-ABC down-regulation in U87 (A) and U251 (C) glioma cells and show percentage of cells infected with MYXV and vMyx-M153KO. HLA-ABC or isotype indicates number of cells stained with HLA-ABC or isotype Ab (detected on the FL2 channel; i =  percentage of cells with Ab staining); GFP indicates number of cells infected with MYXV or vMyx-M153KO (detected on the FLI channel; ii =  percentage of cells infected). Isotype control staining of mock-infected cells was used to set the gating. A representative of at least 3 independent experiments is shown. Flow cytometric analysis of U87 (B) and U251 (D) in triplicates and quantified. Columns represent means of triplicate of one representative experiment of two. Error bars represent 95% confidence intervals, DV =  dead virus, vMyx-M153KO =  mutated MYXV lacking M153R.