Figure 8. MYXV down-regulates MHC I in U87 in vivo.

(A) Immunohistochemistry was performed on paraffin sections of mice brains with established U87 tumors that were treated with indicated viruses for 48 h. Hematoxylin and Eosin (H&E) stain shows tumor, with the arrows indicating tumor location in the brain. Brown M-T7 stain represents MYXV early protein staining within the tumor. Photomicrographs were magnified 25X and 200X. (B) Western Blot of protein extracts from the U87 tumor-bearing hemisphere of mice brains. As control for human HLA-ABC Ab specificity, the contralateral hemisphere without tumor was used. Luciferase expression shows tumor, β-actin serves as protein loading control.