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. Author manuscript; available in PMC: 2013 Jun 10.
Published in final edited form as: Brain Res Mol Brain Res. 2005 Aug 18;138(2):135–144. doi: 10.1016/j.molbrainres.2005.04.015

Fig. 2.

Fig. 2

Tyrosine-phosphorylated tau is enriched in an age-dependent manner. (A) The S1 (soluble) fractions from non-transgenic (NT) and JNPL3 (P301L) mouse brains at 3, 6, and 9 months (M) of age were subjected to immunoprecipitation and Western blot analysis. Tau proteins in these fractions were immunoprecipitated (IP) with either Tau12 or 4G10 antibodies. The immunoprecipitated proteins were analyzed by Western blotting using E1 antibodies. The S1 fractions from both NT and P301L mice were also analyzed by Western blotting using E1 and phospho-tau-specific antibodies PHF1 and CP13. The asterisks denote a non-specific cross-reacting band observed when either PHF1 or CP13 monoclonal antibodies were used. (B) The S1 and P3 fractions, from both NT and JN25 (WT4R) mouse brains, were analyzed by Western blotting, using antibody E1 to determine the abundance of tau proteins at 3, 6, and 12 months of age. (C) The S1 fraction was then subjected to IP using antibody 4G10. The immunoprecipitated proteins were analyzed by Western blotting using antibody E1. The asterisk indicates a non-specific cross-reacting band. The lanes designated Tau6x contain six isoforms of recombinant tau. The filled circles indicate the 0N4R tau isoform.