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. Author manuscript; available in PMC: 2013 Jun 10.
Published in final edited form as: J Am Chem Soc. 2007 Jan 30;129(7):1959–1967. doi: 10.1021/ja065930i

Figure 4.

Figure 4

The optimization and use of DEAL for multiplexed cell sorting. (a & b). Brightfield images showing the efficiency of the homogeneous DEAL cell capture process. (a). A homogeneous assay in which DEAL labeled antibodies are combined with the cells, and then the mixture is introduced onto the spotted DNA array microchip. (b). DEAL labeled antibodies are first assembled onto a spotted DNA array, followed by introduction of the cells. This heterogeneous process is similar to the traditional panning method of using surface bound antibodies to trap specific cells. The homogeneous process is clearly much more efficient. (c). Brightfield and fluorescence microscopy images of multiplexed cell sorting experiments where a 1:1 mixture of mRFP-expressing T cells (red channel) and EGFP-expressing B cells (green channel) is spatially stratified onto spots A1 and C1, corresponding to the encoding of α-CD90.2 and α-B220 antibodies with A1' and C1', respectively. (d). Fluorescence micrograph of multiplexed sorting of primary cells harvested from mice. A 1:1 mixture of CD4+ cells from EGFP transgenic mice and CD8+ cells from dsRed transgenic mice are separated to spots A1 and C1 by utilizing DEAL conjugates α-CD4-A1' and α-CD8-C1', respectively.