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. Author manuscript; available in PMC: 2013 Jun 10.
Published in final edited form as: J Am Chem Soc. 2007 Jan 30;129(7):1959–1967. doi: 10.1021/ja065930i

Scheme 1.

Scheme 1

Illustration of the DEAL method for cell sorting and co-detection of proteins and cDNAs (mRNAs). Antibodies against proteins (for cell sorting) or other proteins (including cell surface markers) are labeled with distinct DNA oligomers. These conjugates may then be combined with the biological sample (cells, tissue, etc.) where they bind to their cognate antigens. When introduced onto a DNA microarray, parallel self assembly, according to Watson-Crick base pairing, localizes the bound species to a specific spatial location allowing for multiplexed, multiparameter analysis.