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. Author manuscript; available in PMC: 2013 Jun 10.
Published in final edited form as: Clin Sci (Lond). 2011 Nov;121(10):427–436. doi: 10.1042/CS20110153

Figure 7. Antenatal CBX consistently increases aortic superoxide production among adult female offspring.

Figure 7

Superoxide production was measured by lucigenin-enhanced chemiluminescence among aorta obtained from adult offspring of dams that received either saline (■) or CBX (□) during pregnancy. These mice were weaned to either regular diet (RD) or hypercaloric diet (HD) until tissue collection. Relative light unit (RLU) production was measured for 7 min with only lucigenin and NADPH, then for 7 min with the aortic segment added, and finally for 14 min after the addition of diphenyleneiodonium (A). NADPH-augmented, diphenyleneiodonium-inhibitable superoxide production was determined by subtracting superoxide production at 28 min from that at 14 min (B). The numbers at the base of the bars represent aggregate numbers. n = 6 female saline–RD, n = 4 female CBX–RD, n = 5 female saline–HD, n = 6 female CBX–HD, n = 2 male saline–RD, n = 5 male CBX–RD, n = 4 male saline–HD and n = 3 male CBX–HD; *P < 0.05 CBX compared with the control.