Figure 5. H459, but not H480 and H481, in the C-terminal domain, is required in Kv3.1b axonal targeting.

A, distribution patterns of Kv3.1bHA and three point mutants in neurons under the permeabilized condition. Anti-HA staining is on the left and anti-MAP2 (microtubule-associated protein 2) staining is on the right. Signals are inverted. B, distribution patterns of Kv3.1bHA and three point mutants in neurons under the non-permeabilized condition. Arrows, axons; arrowheads, dendrites. Scale bars, 100 μm. C, summary of the staining results. One-way analysis of variance followed by Dunn test, **P < 0.01; *P < 0.05.