Figure 6. Biophysical properties of Kv3.1 channels carrying the three histidine mutations in the C-terminus and their response to extracellular zinc.

A, reversible inhibitory effects of extracellular zinc on the currents carried by channels with one to three point mutations. B, summary of effect of zinc on current amplitude of different channel constructs. The numbers of neurons are indicated within the bars, and are the same in B–E. C, summary of zinc's effects on the G–V relationship of three different channel constructs. D, zinc's effect on activation time constants (τ_on). E, zinc's effects on deactivation time constant (τ_off). Paired t-test, **P < 0.01; *P < 0.05.