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. 2013 Feb 18;591(Pt 10):2491–2507. doi: 10.1113/jphysiol.2013.251983

Figure 9. Constructing a fast-spiking neuron with relative resistance to the zinc inhibition.

Figure 9

A, Kv3.1bHAC208A was restricted on somatodendritic membranes in cultured hippocampal neurons revealed by double staining of anti-HA under the non-permeabilized condition and anti-MAP2. B, Kv3.1bHAC208A enriched on somatodendritic membranes. Neurons at 5 DIV were co-transfected with Kv3.1bHAC208A and YFP, fixed 2 days later, and stained with an anti-HA antibody under the non-permeabilized condition. C, Kv3.1bAHAC208A localized to axonal membranes in the presence of KIF5B-YFP. Scale bars, 100 μm. D, summary of axonal targeting of Kv3.1bHAC208A in the absence or the presence of KIF5B-YFP. E, Zn2+ (30 μm) had no clear effect on the action potential firing rate on the neuron expressing YFP at 8 DIV. Square current pulses (1000 ms; 105 pA) were injected into the neuronal soma to induce action potentials. F, Zn2+ (30 μm) significantly reduced spiking frequency of the neuron expressing Kv3.1bHA and KIF5B-YFP. G, Zn2+ (30 μm) had no clear effect on fast spiking of the neuron expressing Kv3.1bHAC208A and KIF5B-YFP. H, summary of the effect of zinc (10, 30 and 100 μm) on spiking frequency. The number of neurons are indicated within parentheses.