Fig. 1.

VN protects against ionizing irradiation-induced cell death in HUVECs. (A) Radiation-induced integrin αv expression. Cells were cultured for 28 h after 8 Gy irradiation. Subsequently, cells in each condition were harvested and whole-cell extracts were subjected to immunoblotting analysis with the indicated antibodies. C, unirradiated control. (B) Cells were exposed to irradiation at a dose of 8 Gy. VN was added to the cell culture 10 min after irradiation, and the cells were continued to be cultured for 28 h. Subsequently, the cells in each condition were harvested, and the number of viable cells was determined by the trypan blue exclusion test using a hemocytometer. *p < 0.05 by Student's t-test. (C) After irradiation, cells were treated with VN, and subsequently harvested as in (B). Whole-cell extracts were subjected to immunoblotting analysis with the indicated antibodies.