Fig. 2.

VN attenuates radiation-induced G1 phase cell cycle arrest through decreasing the expression level of the cell cycle inhibitor protein p21. Cells were exposed to irradiation at a dose of 8 Gy. VN was added to the cell culture 10 min after irradiation, and cells culturing was continued for 28 h. (A) Cells in each condition were subjected to cell cycle analysis using PI staining in accordance with the procedure as described in Section 2. (B) Whole-cell extracts were prepared from HUVECs under the indicated conditions and were subjected to immunoblotting analysis with the indicated antibodies. The relative amounts of p21 protein were quantified and shown as graphs.