Fig. 3.
VN inhibits p21 expression through the ERK and p38MAPK pathways. (A) HUVECs were pre-treated with the indicated inhibitors for 2 h before irradiation, and cell culture was continued for 28 h in the presence of the indicated inhibitors, as shown in Section 2. Whole-cell extracts were subjected to immunoblotting analysis with the indicated antibodies. (B, C) Cells were exposed to irradiation at a dose of 8 Gy. VN was added to the cell culture 10 min after irradiation, and the cell culture was continued for 28 h. Whole-cell extracts were subjected to immunoblotting analysis with the indicated antibodies.