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. 2013 Jun 10;201(6):809–826. doi: 10.1083/jcb.201211105

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© 2013 Kirkland and Kamakaka

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Figure 5. — DSB repair proteins play a role in silencing and localization. (A) Schematic of a cell’s nucleus showing two zones of equal surface area and a GFP focus. (B) Graph of the percentage of cells in each of two zones in a given strain for three independent trials the combined data for the three trials is shown: WT (n = 132); mre11Δ (n = 122), P = 0.27; rad51Δ (n = 134), P = 0.90; and ku70Δ/esc1Δ (n = 197), P = 5.8 × 10⁻⁷. Data shown are for all three trials combined, and p-values were determined by χ² test compared with WT (see Materials and methods). The ku70Δ/esc1Δ data were previously published (Ruben et al., 2011) and are shown here for ease of comparison. Representative images of the two zones are provided under the graph. Bar, 1,000 nm. (C) Schematic of a cell’s nucleus showing two zones of equal volume and a GFP focus. (D) Graph of the percentage of cells in each of two zones of equal volume in a given strain using the data from B: WT (n = 132); mre11Δ (n = 122), P = 0.46; rad51Δ (n = 134), P = 0.58; and ku70Δ/esc1Δ (n = 197), P = 2.7 × 10⁻⁸. The ku70Δ/esc1Δ data were previously published (Ruben et al., 2011) and are shown here for ease of comparison. (E) 10-fold dilutions of α strains on a YPD plate (growth control) or an a lawn to assay silencing at HMR (left) or 10-fold dilutions of a strains on a YPD plate (growth control) or an α lawn to assay silencing at HML (right).