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. 2013 Jun 10;201(6):809–826. doi: 10.1083/jcb.201211105

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© 2013 Kirkland and Kamakaka

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Figure 6. — γ-H2A is enriched at HM loci and their surrounding chromosomal regions. (A) Schematic of the HML and HMR loci on chromosome III with amplicons used in ChIP-qPCR. Schematic is not to scale. (B) ChIP mapping of γ-H2A. Data are presented as the mean enrichment of IP/input further normalized to an ACT1 amplicon for six IPs from three independent cross-links. Error bars are standard deviation from the mean. (C) γ-H2A enrichment normalized to H3 enrichment to consider nucleosome occupancy. All amplicons normalized to the ACT1 locus.