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. 2013 Jun 10;201(6):809–826. doi: 10.1083/jcb.201211105

Figure 7.

Figure 7.

The heterochromatin silencer is necessary for γ-H2A enrichment at HMR, and the tDNA insulator restricts the spread of γ-H2A from HMR. (A) ChIP-qPCR plots of γ-H2A enrichment at HMR in an hmr-eΔ strain. (B) γ-H2A/H3 enrichment at HMR in the hmr-eΔ strain. (C) γ-H2A enrichment at HMR in a tT(AUG)CΔ strain (D) γ-H2A/H3 enrichment at HMR in the tT(AUG)CΔ strain. Plots are a mean of three cross-links and six IPs. All amplicons normalized to the ACT1 locus. WT data are the same as in Fig 6 and are included for ease of comparison. Error bars are standard deviation from the mean. P-values by t test are assigned as ***, P < 0.001; **, P < 0.01; and *, P < 0.05.