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. 2013 Jun 10;201(6):809–826. doi: 10.1083/jcb.201211105

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© 2013 Kirkland and Kamakaka

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Figure 8. — SMC proteins are enriched at HMR and Scc2 enrichment requires γH2A. (A) Schematic of chromosome III qPCR amplicons. (B–D) ChIP-qPCR enrichment of Mcd1-myc (cohesin; B), Brn1-HA (condensin; C), and SMC6-TAP (reparin; D). (E) ChIP-qPCR plots of Scc2 in a WT and htaS129* mutant. Plots are a mean of at least two independent cross-links and four IPs. Error bars are standard deviation from the mean. P-values by t test are assigned as ***, P < 0.001; and *, P < 0.05.