Fig. 2.

RBC were labeled with biotin and reinfused into a normal subject. At the times indicated labeled cells were isolated with streptavidin-coated magnetic beads and the fraction HbA1c determined by HPLC. The mean RBC age was calculated for each time point (inverted triangles) according to [45]. The open square represents the value for TfR+ reticulocytes isolated with anti-TfR-coated magnetic beads and assayed the same way. These data indicate that the amount of HbA1c increases linearly as the RBC age. This research was originally published in Blood in 2008 [1].