Figure 2.

IL-6 is indispensable for development of IL-22-producing CD4⁺ T cells

(A) Naïve OTII-Tg CD4 T cells were sorted and activated by Ova peptide for 3d using splenic APCs from Il23a^−/− or Il6^−/− mice, with or without addition of the indicated neutralizing mAbs. Recovered cells were stained for surface CD4 and intracellular IL-22 and analyzed by flow cytometry. Numbers indicate the frequencies of IL-22⁺ CD4⁺ T cells.

(B, C) Pooled frequency data from three or more independent experiments performed as in (A). Data are means +/− SEM (*p<0.05, **p<0.01).