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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: J Neuroimmunol. 2012 Jul 4;251(0):33–38. doi: 10.1016/j.jneuroim.2012.06.006

Figure 3. JAK2 phosphorylation in IFN-γ-treated neurons is significantly extended as compared to MEF.

Figure 3

Neurons and MEF were treated as described (Figure 1), and equal volumes of cell lysates were examined by immunoblotting for a) phospho-JAK2 (pY1007/1008); b) total JAK2; and GAPDH signals. c) Blot signals were quantified using densitometry, and phospho-JAK2 signals were normalized to total JAK2 expression and to GAPDH for loading. Values are expressed as a percent of control. Data shown are from a representative experiment. Ø: cells not exposed to IFN-γ.