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12S-hydroxy-1,12-pyrazolinominocycline (PMIN; 0.95, 9.50 or 95.00 μM) inhibits LPS-induced prostaglandin (PG) E2 microglial production, as assessed by enzyme immunoassay. PMIN (or vehicle) was added to the cell media 30 min prior to LPS (10 ng/ml, 24-h incubation). Data are expressed as mean ± S.E.M. ** p < 0.01 compared with LPS control.
