Table 1.
List of aging and exposure history independent urinary radiation biomarkers
| RT (min)a |
m/z (ESI mode) |
Identity | Mass error (ppm) |
RT error (min) |
MRM (ionization mode)b |
Trendc | Metabolic pathway |
Sensitive to |
|---|---|---|---|---|---|---|---|---|
| 0.6 | 251.065+ | 2’-Deoxyuridine + Na+ | 4 | 0.0 | 227→ 184 (−) | ↑ | Pyrimidne metabolism | Aging |
| 0.3 | 188.175+ | N1-Acetylspermidine + H+ | 3 | 0.0 | 188→ 100 (+) | ↑ | Polyamine metabolism | Aging |
| 1.0 | 265.081+ | Thymidine + Na+ | 5 | 0.0 | 241→ 151 (−) | ↑ | Pyrimidne metabolism | Prior exposure |
| 0.3 | 228.097− | 2’-Deoxycytidine– H+ | 3 | 0.0 | 228→ 112 (−) | ↑ | Pyrimidne metabolism | Prior exposure |
| 0.4 | 151.026− | Xanthine – H+ | 0 | 0.1 | 151→ 107 (−) | ↑ | Purine metabolism | Aging |
| 0.5 | 227.066− | 2’-Deoxyuridine – H+ | 5 | 0.1 | 227→ 184 (−) | ↑ | Pyrimidne metabolism | Aging |
| 1.0 | 241.082− | Thymidine – H+ | 4 | 0.0 | 241→ 151 (−) | ↑ | Pyrimidne metabolism | Prior exposure |
| 0.8 | 283.066− | Xanthosine – H+ | 8 | 0.1 | 285→ 153 (+) | ↑ | Purine metabolism | Aging |
a
All RT (retention time) in reverse-phase global analysis.
b
Characteristic fragmentation reactions monitored for quantitation via multiple reaction monitoring (MRM).
c
All trends indicate statistically significant and consistent change after quantitation and normalization with respect to creatinine clearance.