Table 3.
Effect of leptin infusion (4 μg/kg/min, IP) for 7 days in female Stat3flox/flox control and Stat3flox/flox/POMC-Cre mice at 20 weeks of age
| Parameter | Stat3flox/flox Female | Stat3flox/flox POMC-Cre Female | Leptin in Stat3flox/flox Female | Leptin in Stat3flox/flox POMC-Cre Female |
|---|---|---|---|---|
| Body Weight (g) | 27.4 ± 1.1 | 32.0 ± 0.8* | 23.0 ± 0.7# | 29.3 ± 0.8# |
| Food Intake (g) | 3.4 ± 0.3 | 4.4 ± 0.3* | 2.7 ± 0.3# | 3.0 ± 0.3# |
| Leptin (ng/mL) | 6 ± 3 | 15 ± 4 | 53 ± 16# | 64 ± 9# |
| Insulin (μU/mL) | 12 ± 2 | 15 ± 2 | 14 ± 9 | 20 ± 5 |
| Glucose (mg/dL) | 199 ± 23 | 175 ± 19 | 141 ± 12# | 169 ± 10 |
| RQ (VCO2/VO2) | 0.83 ± 0.05 | 0.82 ± 0.02 | 0.79 ± 0.03# | 0.76 ± 0.03# |
| VO2 (ml/kg/min) | 73.9 ± 4.7 | 69.7 ± 3.9 | 76.0 ± 6.8 | 65.0 ± 3.6 |
| Motor Activity (m/d) | 82 ± 16 | 130 ± 25* | 44 ± 11# | 136 ± 30 |
| Heat Production (cal/hr) | 558 ± 43 | 623 ± 36 | 500 ± 46 | 581 ± 32 |
| MAP (mmHg) | 111 ± 5 | 114 ± 3 | 121 ± 9# | 115 ± 4 |
| HR (bpm) | 560 ± 15 | 603 ± 11* | 578 ± 20 | 608 ± 6 |
RQ, VO2, Motor Activity, Heat Production, MAP, and HR represent the average values of 3 consecutive days at the end of the control (open) and leptin infusion periods (shaded). Data are expressed as mean ± SEM.
*
p<0.05, Stat3flox/flox/POMC-Cre mice vs. sex-matched Stat3flox/flox control mice.
#
p<0.05, leptin infusion vs. control period in sex-matched mice. RQ indicates respiratory quotient; VO2 indicates oxygen consumption.