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Phosphorylation of Ser161 and Ser165 does not affect JAK2-mediated phosphorylation of SH2B1β at Tyr439 and Tyr494. Cell lysates from 293T cells expressing the indicated GFP-SH2B1β constructs and FLAG-JAK2 were immunoprecipitated with αGFP. Lysates or immunoprecipitation samples were immunoblotted with the indicated antibodies; n = 2.
