Table 2.

Primers and probes for quantitative real-time PCR assays of rainbow trout gene transcription for candidate genes chosen for a temperature and immune challenge

Gene	Treatment	Accession	Forward seq	Reverse seq	MGB probe seq	Reference
EF-1α	Reference	AF498320	AATACCCTCCTCTTGGTCGTTTC	CTTGTCGACGGCCTTGATG	TGCGTGACATGAGGC	Aykanat et al. (2011)
ARP	Reference	AY685220	TTGTTTGACTAACTTGCTATTCTTTGC	CGCCGACAATGAAACATTTG	AATTGCTGGATGACTATC	Ortega et al. (2005)
CathepsinD	Temperature	U90321	GGGAGGAACTGACCCGAAGT	GCGGCTGACGTCGAGGTA	CTACAGTGGAGACTTCCA	This paper
GR	Temperature	Z54210	CTGGCTGTTCCTCATGTCGTT	CAACATCCCCCCGTTACACT	CTTGGGCTGGCGCT	This paper
PEPCK	Temperature	AF246149	GCCCCTTCTTCGGCTACAA	CTTGCGGGTCTCCATGCT	TCGGTGACTACCTAGCC	This paper
PK	Temperature	AF246146	TGGGCCGACGATGTAGACA	CCCCTGGCCTTTCCTATGTT	CAGAGTCAACTTCGGC	This paper
IL-1β	Immune	AJ223954; AJ298294	ACATTGCCAACCTCATCATCG	TTGAGCAGGTCCTTGTCCTTG	ATGGAGAGGTTAAAGGGT	Raida and Buchmann (2008)
CXCL-8	Immune	AJ279069	AGAATGTCAGCCAGCCTTGT	TCTCAGACTCATCCCCTCAGT	TTGTGCTCCTGGCCCT	Raida and Buchmann (2008)
IFNγ	Immune	AY795563	CAAACTGGCCCTTAAGTTCCA	TCTGGGCTTGCCGTCTCT	TAAAGAAGGACAACCGCAGG	T. Aykanat, unpubl. data
TNFα	Immune	AJ277604; AJ401377	GGGGACAAACTGTGGACTGA	GAAGTTCTTGCCCTGCTCTG	ACCAATCGACTGACCGAC	Raida and Buchmann (2008)

PCR, polymerase chain reaction; GR, glucocorticoid receptor; PEPCK, phosphoenolpyruvate carboxykinase; PK, pyruvate kinase; IL-1β, interleukin 1β; CXCL-8, interleukin 8; IFNγ, interferon γ; TNFα, tumor necrosis factor α.