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Inhibitory effect of ATG (10 μM) on tyrosinase gene expression was analyzed by real-time qPCR (a) and western blot (b) in B16BL6 cells stimulated with α-MSH. The effect of ATG (1, 10, 20, and 50 μM) on tyrosinase promoter activity was determined using the luciferase reporter system (c). Dose-dependent inhibitory effect of ATG (1, 10, and 50 μM) was observed in the CRE-luciferase assay (d), and the level of P-ERK increased in a time-dependent manner (e).
