Skip to main content
. Author manuscript; available in PMC: 2013 Jun 11.
Published in final edited form as: J Virol Methods. 2011 Feb 3;173(2):378–383. doi: 10.1016/j.jviromet.2011.01.021

Fig. 1.

Fig. 1

Instability of Ty/Ont-PB2 in a standard reverse genetics plasmid. (A) Diagram of the PB2 gene segment (positive sense) of Ty/Ont and Mal/WI illustrating the position of their six amino acid differences. (B) Agarose gel electrophoresis of PB2 genes of Ty/Ont (T) or Mal/WI (M) after amplification by RT-PCR, and the StuI linearized reverse genetics plasmid pG26A12 (P). L, 1kb plus DNA marker ladder (Invitrogen). (C) Colonies generated from Ty/Ont-PB2 recombination reaction. (D) Colonies generated from Mal/WI-PB2 recombination reaction. (E–F) Representative agarose gel electrophoresis of PCR amplicons obtained from colonies derived from the (E) Ty/Ont-PB2 recombination reaction (none contained an insert of correct length), or the (F) Mal/WI-PB2 recombination reaction (all contained an insert of correct length).