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. Author manuscript; available in PMC: 2013 Jun 11.
Published in final edited form as: J Virol Methods. 2011 Feb 3;173(2):378–383. doi: 10.1016/j.jviromet.2011.01.021

Fig. 4.

Fig. 4

Ty/Ont-PB2 was cloned successfully into the new low-copy reverse-genetics plasmid pGJ3C3. (A) The colonies derived from transformation with the Mal/WI-PB2 recombination reaction were large and uniform. (B) The majority of the colonies derived from the Ty/Ont-PB2 recombination reaction were small; however, a few large colonies (arrows) were present. (C) Agarose gel electrophoresis of amplicons from the PCR screen of colonies from the Ty/Ont-PB2 recombination reaction showed that the large colonies did not contain full-length PB2, whereas, the small colonies contained full-length PB2 inserts (2.3 kb). The length of specific fragments of the 1kb plus DNA marker ladder (L) are indicated for reference.