Table 2.

Modest Decrease in Number of Hepatocytes in IFNγ-Injected Larvae

	Non-2F11+	PCNA+ non-2F11+	Fraction PCNA/2F11	Ratio 2F11+/non-2F11+
3 dpf control	202.5±37.4	189.8±35.9	0.94±0.02	0.05±0.02
3 dpf IFNγ-injected	172.2±62.0	158.5±56.1	0.92±0.04	0.04±0.01
4 dpf control	356.8±36.1	276.8±19.6	0.78±0.07	0.08±0.01
4 dpf IFNγ-injected	265.8±48.5**	199.5±37.3**	0.75±0.07	0.05±0.02*

In the same 6 larvae shown in Table 1, non-2F11-expressing cells±PCNA were counted. Of note, the total number of cells counted in the livers is the sum of the 2F11+ and non-2F11+ cells. At 3 dpf, there is not a statistically significant difference between non-2F11+ cells (mostly hepatocytes) in control and in IFNγ-injected larvae. At 4 dpf, there is a difference between the number of hepatocytes and proliferating hepatocytes in control and in the IFNγ-injected larvae. The last column shows the ratio of cholangiocytes to noncholangiocytes in the livers, showing that at 4 dpf there are proportionally fewer cholangiocytes in the IFNγ-injected larvae. (^*p≤0.05; ^**p≤0.005, all relative to control for the same age; other comparisons not significant; comparisons by Student's t-test).