Figure 3.

Representative photograph showing the effect of neem leaf extract (NLE) (50 mg/day for 10 days) on immunocytochemical localization of inducible nitric oxide synthase (iNOS) expression and total nitrite concentration in granulosa cells of preovulatory COCs. Most of the granulosa cells collected from preovulatory COCs of NLE-treated animal show higher iNOS expression as compared to control (a; arrows). NLE treatment increased total nitrite concentration in granulosa cells of preovulatory COCs (b). Data are mean ± SE of three replicates. *Denotes significantly (P < 0.05) higher as compare to control (n = 3/group; Student's t-test). Bar = 40 μm