Figure 7.

Representative photograph showing neem leaf extract (NLE) (50 mg/day for 10 days) induced deoxyribonucleic acid (DNA) fragmentation in granulosa cells encircling oocytes that showed normal morphology at the time of collection. (a) Granulosa cells encircling preovulatory oocytes did not show DNA fragmentation as evidenced by green fluorescence of acridine orange (AO). (b) NLE-induced apoptosis in most of the granulosa cells encircling preovulatory oocyte as evidenced by yellowish orange fluorescence of ethidium bromide (EB) (arrows). (c) The initiation of apoptosis was also observed in granulosa cells encircling ovulated oocyte as evidenced by yellow fluorescence of EB (arrow). (d) NLE treatment induced cell death thereby reduced number of granulosa cells encircling ovulated oocyte as evidenced by reddish fluorescence of EB (arrows) (n = 3/group). Bar = 40 μm