Figure 3.
Photoprobe 24 (CCG-206559) retains biological activity to block prostate cancer migration. A. Cellular migration determined by wound assay. PC-3 prostate cancer cells were grown to confluence in 12-well plates and a scratch was made with a 200 µL pipette. Images were taken at the beginning of the experiment and after 24 h in DMEM containing 0.5% FBS and the indicated concentration of 24 or 0.1% DMSO. After 24 h the cells were stained with crystal violet to produce high-contrast images. Shown are examples of 10 μM 24 and DMSO after 24 h, from experiments with similar initial wound areas as determined by using image analysis software. B. Quantification of wound assay migration. The change in migration over 24 h was determined by the difference between the area of the wound after 24 h and the initial area of the wound. The percent inhibition was plotted by normalizing the compound-treated cells to the DMSO control. Results are expressed as the mean (±SEM) of triplicate experiments. A nonlinear least-squares regression curve was fit for CCG-206559 inhibition of migration, IC50 = 4.7 μM.