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. 2013 Mar 1;5(5):1615–1620. doi: 10.3892/ol.2013.1223

Figure 2.

Figure 2

Key role of caspase 3 in regulating the growth stimulating signal released from dying ECs. (A) Blocking effects of selective caspase 3 and caspase 6 inhibitors on the growth stimulating effect of dying ECs on U87MG cells (*P<0.05). Z-DEVD-FMK, a caspase 3 inhibitor (100 μM), or Z-VEID-FMK, a caspase 6 inhibitor (100 μM) were added into the 24-well plates. (B) Western blot analysis of caspase 3 and caspase 6 expression levels in dying ECs following various treatments. shRNA without a target (shNT) was used as a negative control. (C) Knockdown of caspase 3 significantly inhibited the growth promoting effect of dying ECs on U87MG cells (*P<0.05). (D) Knockdown of caspase 6 showed no effect on the growth stimulating signal from dying ECs. All error bars repesent SEM, n=4. EC, endothelial cell.