Figure 3.

Involvement of iPLA₂ in caspase 3-mediated growth stimulating pathway in dying ECs. (A) Immunoblot analysis of iPLA₂ expression in dying ECs following PLA2G6 knockdown. (B) Growth stimulating effect of dying ECs on U87MG cells was reduced by knockdown of PLA2G6 (^*P<0.05, between days 2 and 8). (C) Expression levels of ac iPLA₂ in ECs following PLA2G6 overexpression. (D) Overexpression of PLA2G6 in ECs significantly promoted the proliferation of U87MG cells compared with wild type ECs (^*P<0.05, between days 2 and 8). All error bars represent SEM, n=4. iPLA₂, calcium-independent phospholipase A₂; EC, endothelial cell; ac, active.