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. Author manuscript; available in PMC: 2013 Jun 11.

Published in final edited form as: Antivir Ther. 2010;15(2):253–265. doi: 10.3851/IMP1512

Expression pattern of (A) cytokeratin 5 and (B) cytokeratin 14 in untreated and Amprenavir treated gingival raft cultures. Primary gingival keratinocytes were grown in organotypic (raft) cultures and treated with different concentrations of Amprenavir at day 8. (Panels a, g and m) untreated rafts; (Panels b, h and n) rafts treated with 2 μg/ml Amprenavir; (Panels c, i and o) rafts treated with 5 μg/ml Amprenavir; (Panels d, j and p) rafts treated with 7.66 μg/ml Amprenavir; (Panels e, k and q) rafts treated with 9 μg/ml Amprenavir; (Panels f, l and r) rafts treated with 12 μg/ml Amprenavir. Rafts were harvested at different points and stained with anti-cytokeratin 5 and 14 antibody. (Panels a to f) rafts were harvested at 2 days post treatment; (Panels g to l) rafts were harvested at 4 days post treatment; (Panels m to r) rafts were harvested at 6 days post treatment. Arrows indicate the expression of cytokeratin 5 and 14. Images are at 10 X original magnification. DPT denotes days post treatment.

Expression pattern of (A) cytokeratin 5 and (B) cytokeratin 14 in untreated and Amprenavir treated gingival raft cultures. Primary gingival keratinocytes were grown in organotypic (raft) cultures and treated with different concentrations of Amprenavir at day 8. (Panels a, g and m) untreated rafts; (Panels b, h and n) rafts treated with 2 μg/ml Amprenavir; (Panels c, i and o) rafts treated with 5 μg/ml Amprenavir; (Panels d, j and p) rafts treated with 7.66 μg/ml Amprenavir; (Panels e, k and q) rafts treated with 9 μg/ml Amprenavir; (Panels f, l and r) rafts treated with 12 μg/ml Amprenavir. Rafts were harvested at different points and stained with anti-cytokeratin 5 and 14 antibody. (Panels a to f) rafts were harvested at 2 days post treatment; (Panels g to l) rafts were harvested at 4 days post treatment; (Panels m to r) rafts were harvested at 6 days post treatment. Arrows indicate the expression of cytokeratin 5 and 14. Images are at 10 X original magnification. DPT denotes days post treatment.