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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Nat Genet. 2013 Mar 24;45(5):478–486. doi: 10.1038/ng.2591

Figure 3. Recurrent somatic alterations in ELMO1, DOCK2, and other RAC1 Guanine Nucleotide Exchange Factors (GEFs).

Figure 3

a) Schematic of protein alterations in DOCK2 and ELMO1 detected by WES. Coding alterations in EAC are colored either black (missense) or red (splice site/nonsense); silent mutations are depicted in gray. Conserved domain mapping is from UniProt; SH3, SRC Homology 3; DHR, Dlg homologous region, ELMO, Engulfment and Cell Motility; PH, Pleckstrin homology. b) Sample frequency (left) of candidate ELMO1 and DOCK2 as well as other Rac1-activating guanine nucleotide exchange factors in 145 WES EACs. c) ELMO1 wild-type or mutants (or GFP control) were expressed in NIH/3T3 cells using retroviral transduction with the pBabe vector. Cells were plated in matrigel invasion chambers with full serum containing medium in the lower chamber only, and invading cells from four fields were counted. Invading cells of 3 independent replicates are shown. Error bars represent S.D. P-values compare mutant ELMO1 to wild-type. n.s., not significant. Student’s t-test.