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PCR analysis of the MMP-7 allele in 7ko and WT cell lines. Bands of 535 bp and 685 bp were expected for 7ko and WT cell lines, respectively (A). No immunostaining for MMP-7 was observed in WT or 7ko immortalized fibroblasts [(B) and (C); PI nuclei, red], and MMP-7 was localized to the cytoplasm of the immortalized WT corneal epithelial cells (D).
