Immunoblot analysis of MMP-7 expression in media (lanes 1–3) and lysates (lanes 4–6) from WT, 7ko, and 7ko-MMP-7 fibroblasts (A). The 7ko and WT groups served as negative controls. Immunoblot analysis of collagen XVIII NC1 degradation induced by media collected from 7ko, 7ko-vector, and 7ko-MMP-7 fibroblast cultures (B). Three independent experiments were performed to quantify the intensity of remaining, unprocessed NC1 fragments. The NC1 fragment density in the 7ko-MMP-7 group is 0.72 ± 0.15% of that of the control 7ko group (C).