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. Author manuscript; available in PMC: 2013 Jun 11.
Published in final edited form as: Methods Mol Biol. 2011;758:215–235. doi: 10.1007/978-1-61779-170-3_15

Fig. 2.

Fig. 2

The HT-22 cell line exhibits an increase in mitochondrial activity upon exposure to galactose. The HT22 mouse neuroblastoma cell line was exposed to varying concentrations of galactose for 48 h and reducing capacity of the cells was assessed using an MTT assay. Complete replacement of glucose with galactose (100%) led to an increase in MTT absorbance due to enhanced mitochondria activity. Slow metabolism of galactose altering viability and ATP production reveals underlying dependence upon oxidative phosphorylation. This data suggests that even with half the normal amount of glucose, HT-22 cells can successfully produce sufficient ATP for survival and proliferation.